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BI-122 Biology
Prokaryotes
General Characteristics
  - Cells bounded by plasma membrane
 
  
    - cell walls, various materials
 
    - gelatinous capsule, various materials
 
    - classified by chemical characters
 
    
  - absence of membrane-bound organelles
    
infolding of cell membrane probably serves organelle function 
  - absence of nuclear membrane;
    
presence of central nuclear region 
  
    - single, circular double stranded DNA molecule ['chromosome']
      
without associated chromatin protein [typical of eukaryotic chromosomes] 
    - few to numerous plasmids
      
consist of short DNA fragments 
    - asexual reproduction
 
    
      - reorganization of nuclear region to separate DNA into two nuclear regions
 
      - cell fission
 
      
    - no sexual reproduction
      
exchange of genetic information occurs: 
    
      - conjugation = sharing of DNA material by merging two cells together
 
      - transformation = collect plasmids from dead cells in medium
 
      - transduction = transfer of plasmids between cells by bacteriophage virus
 
      - plasmids may be incorporated into circular DNA molecule
 
      
    
  
Classification
Archaea [archaebacteria]
  - Most primitive organisms known
 
  - may still be forming near volcanic 'black smokers' along Mid-Atlantic rift
 
  - unique sequences of bases in RNA
 
  - chemosynthetic
 
  
    - methane bacteria
 
    
      - obligate anaerobes
 
      - common in benthic deposits, hot springs, fecal material, decaying organisms,...
 
      - derive energy from methane [marsh gas] production
        
CO2 + 4H+ → CH4 + 2O-2 
      
    - Salt bacteria
 
    
      - photosynthetic, using bacterial rhodopsin, red in color
        
does not produce waste O2 
      - found in brine [Great Salt Lake, Dead Sea, desalination plants,...]
 
      
    - Sulfolobus bacteria
 
    
      - derive energy by changing ionization state of sulfur
        
sulfurous to sulfuric, yielding H2S [rotten egg smell] 
      - found in geothermal sulfur hot springs, embers of coal tailing, 
        perhaps coal deposits,...
 
      - ribosomes are unusual shape (and smaller) compared to other organisms
 
      
    
  
Bacteria, or true bacteria
  - Class Bacteriophyta
 
  
    - heterotrophic free-living
      
saprophytes, on decaying organisms 
    - heterotrophic parasites
      
including pathogenic types 
    - autotrophic, free-living
 
    
      - purple sulfur bacteria, with bacteriochlorophyll, or b-chlorophyll
        
CO2 + 2H2S → (CH2O)n 
          + H2O + 2S 
      - purple nonsulfur bacteria, with bacteriochlorophyll, or b-chlorophyll
        
CO2 + 4H+ → (CH2O)n 
          + H2O 
      - green sulfur bacteria, with unique chlorobium chlorophyll
        
CO2 + 2H2S → (CH2O)n 
          + H2O + 2S 
      - Iron bacteria, change ionic state of Fe (ferrous to ferric)
 
      - Sulfur bacteria
        
hydrogen sulfide to elemental sulfur
        
elemental sulfur to sulfate (sulfurous) 
      - Hydrogen bacteria, molecular hydrogen to ionic hydrogen
 
      - Nitrifying bacteria, molecular N2 to nitrites (nitric)
 
      - Nitrogen fixing bacteria, nitires to nitrates (nitrous)
        
frequently in root nodules of Legumes 
      
    
  - Class Cyanobacteria [blue-greens, formerly blue-green algae]
 
  
    - chlorophyll a, as in green Protists and Plants
 
    - phycobilins
      
blue phycocyanin
      
red phycoerythrin 
    - produce waste O2
 
    - alternate pathway: nitrogen fixing
 
    - found in non-acidic fresh water, salt water (marine), hot springs [Yellowstone],
      frozen lakes and glaciers (Antarctic and Arctic regions)
 
    
  - Class Prochlorobacteria [bright green]
 
  
    - discovered in 1976 by R. A. Lewin, Scripps Institute of Oceanography
      
shallow marine waters, off the coast of Baja California 
    - chlorophyll a and chlorophyll b, as in green Protista and Plants
 
    - no phycobilins, but caretinoid pigments as in Plants
 
    
  
  
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  revised 19 aug 2006