DNA Hybridization


The first step in hybridization is to separate the two strands of DNA from two samples,
then mix the separated strands.

  • each strand tends to combine with complement
    . . hydrogen bonding
    . . . A-T and T-A
    . . . G-C and C-G
  • regardless of source
    . . - expect nearly 100% pairing
    . . . .if both samples from same source
    . . - expect percentage pairing to equal percent similarity
    . . . .if samples from multiple sources

    DNA Hybridization has been used in studies of phylogeny

  • closely related species should show high hybridization
  • distantly related species should show low hybridization
  • Therefore, DNA hybridization percentage estimates evolutionary relationship

    PANDA PHYLOGENY
    - - - bear
    - giant panda
    - - red panda
    - raccoon


    "huffalump" PHYLOGENY
    \ \ - - Mastodon
    \ - - Mammoth
    \ - - Indian elephant
    - - African elephant

    "Huffalumps and woosels" -A.A. Milne, Winnie, the Pooh



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    © 2004 Prof. LaFrance, Ancilla College